ALiCE® Cell-Free Protein Expression Maxi Kit (60 reactions)

Expression Systems

Cell-Free Protein Expression System

Germany

The protein produced with the ALiCE Cell-Free Protein Expression kit can be used for any application, including: Structural analysis experiments (including NMR and X-ray crystallography​), Protein interaction studies​, Enzyme assays​, Membrane protein expression and analysis​, Protein activity screening​, Cryogenic electron microscopy

The ALiCE kits were developed specifically for ease of use. Expression of your protein of interest follows a simple four-step procedure: 1. Clone your gene of interest into the ALiCE vector of your choosing 2. Add your plasmid DNA to a thawed vial of ALiCE lysate 3. Incubate at 25°C with aeration for 24-48 hours. 4. Collect your protein For the full details of the procedure, download our user manual.

UN1845

Class 9 (Dry Ice)

III (Dry Ice)

UN 1845 (Dry Ice)

The ALiCE® Cell-free protein expression kits are intended for research use only. It is not to be used for diagnostic or preventive action or treatment of a disease, nor to be administered to humans. Please refer to the Limited Use Label License (LULL) in the Documents section.

We assume no liability for any direct or indirect damages or loss arising from the use, misuse, or results of use of this kit. The user should observe all applicable regulations for handling chemicals and recombinant DNA. Lab coat, safety glasses and gloves should be worn when handling the kit components. When handling ultracold material wear additional protective gloves to avoid frostbite. Used components are to be disposed of in accordance to the local regulations.

Liquid (Frozen)

Published examples of proteins expressed in ALiCE® includes: Membrane proteins, Antibodies, Protein complexes (e.g. VLPs), Growth factors.

Plant-based cell-free lysate derived from Nicotiana. tabacum c.v. BY-2 root cells.

High quality chemicals and materials have been used to manufacture the components of this kit. Each lot is carefully tested to ensure that all components meet the stated specifications (see Certificate of Analysis). Every batch of the ALiCE lysate undergoes rigorous quality control testing. Before release, the lysate must meet our stringent criteria of achieving 3 mg/mL eYFP following a 48 hour reaction period.

The kit contains reagents for 60 x 50 µL ALiCE protein expression reactions: • 6 x 500 µL ALiCE lysate aliquots • pALiCE01 vector • pALiCE02 vector • 60 x ALiCE reaction tubes • Short protocol

• Gloves • Pipette filter tips (RNase-free) • RNase-free water • Plasmid purification kit for isolating RNase-free transfection-grade plasmid DNA. We recommend using the Macherey-Nagel™ NucleoBond™ Xtra kit. • For reactions performed in tubes: Eppendorf ThermoMixer® series or similar heated table-top shaker with an equivalent shaking diameter of 3 mm. • Recommended For reactions performed in microtiter plates: 96-well half area microplates and a shaker system with a humidity above 70%, run at 500 rpm with a shaking diameter of 12.5 mm. Additionally, we recommend using the Sandwich cover system “Duetz” from EnzyScreen BV (Heemstede, Netherlands) together with their universal clamp holder system. Please follow the recommendations for the microtiter plate setup if humidity control is missing. • For proteins expressed in the microsome: n-dodecyl-β-maltoside (DDM)

TAATACGACTCACTATAGGGAGAGTATTTTTACAACAATTACCAACAACAACAACAAACAACAACAACATTACAT TTTACATTCTACAACTACCATGGCTTGGTCTCATCCGCAATTCGAAAAAAGCGCTGAAAACCTGATCGAAGGCCG TGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCA CAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCAC CGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCC CGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTT CAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCT GAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGT CTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAG CGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTA CCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGAC CGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAATAAGGTACCAAGCTCTTCTGGTTTGGTTTGGA CCTCTGGTCCTGCAACTTGAGGTAGTCAAGATGCATAATAAATAACGGATTGTGTCCGTAATCACACGTGGTGCG TACGATAACGCATAGTGTTTTTCCCTCCACTTAAATCGAAGGGTTGTGTCTTGGATCGCGCGGGTCAAATGTATA TGGTTCATATACATCCGCAGGCACGTAATAAAGCGAGGGGTTCGAATCCCCCCGTTACCCCCGGTAGGGGCCCAT TATAGCCGAATTCGGCGCGCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTA AATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAG TATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCC AGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAA CAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATG TGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTT GGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCAT AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTT GCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGA GCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGC TTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGC TGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGA TGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAT CGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGA TTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTA ACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCT GCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACC AACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTT AGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGC CAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTG AACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCT ATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGA GCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGA GCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTT CCTGGCCTTTTGCTGGCCTTTTGCTCACATG

ALiCE® is a plant-based cell-free lysate derived from Nicotiana. tabacum c.v. BY-2 root cells. ALiCE® stands for Almost Living Cell-Free Expression. The lysate is prepared by removing the cell wall and other cell components that are not necessary for protein production, leaving only the the protein production machinery and mitochondria which power the reaction. During our lysate finalization steps, the additional components necessary for protein production (amino acids, co-factors, etc.) are added to produce something that functions just like a cell, but without the constraints of a living system - all that's needed is the DNA encoding the protein of interest!

ALiCE® is a registered trademark of LenioBio GmbH in Germany