Description
HEMOXCell®, a patented technology, is a marine macro hemoglobin able to carry 156 molecules of oxygen. HEMOXCell® is a ready-to-use solution. The hemoglobin catches the oxygen and releases it according to the P02 gradient (the demand of oxygen). The link with oxygen, or formation of oxyhemoglobin, is a cooperative, allosteric process where the hemoglobin-oxygen link is a ected by the saturation of oxygen. HEMOXCell® also has anti-oxidative properties (SOD activity) which help to maintain cell culture media quality.
HEMOXCell®, a patented technology, is a marine macro hemoglobin able to carry 156 molecules of oxygen. HEMOXCell® is a ready-to-use solution. The hemoglobin catches the oxygen and releases it according to the P02 gradient (the demand of oxygen). The link with oxygen, or formation of oxyhemoglobin, is a cooperative, allosteric process where the hemoglobin-oxygen link is a ected by the saturation of oxygen. HEMOXCell® also has anti-oxidative properties (SOD activity) which help to maintain cell culture media quality.
Specifications
CLASS
Cell Culture Supplements & Reagents
SHIPPING NOTES
Classified as “Biorisk” in packaging and transport regulation UN 3373
Not a dangerous product. In agreement with directive 1999/45/CE, the product has to be identified and classified as “Biorisk”.
USAGE
Cell Growth Activator
*USAGE / SAFETY STATEMENT
For R&D use only. Not for human or animal therapeutic or diagnostic use.
CONCENTRATION
The optimum concentration defined with CHO-320, for example, is: 0.025 mg/ml. One sample of HEMOXCell® 1 (see table below) can be tested in 2 L of media at 0.025 mg/ml
FREE CHARACTERISTICS
Biological product whose origin is a marine invertebrate and is therefore animal free HEMOXCell® is a macromolecule of 3.6 Mda
APPEARANCE
Red solution, liquid or frozen
MICROPLATE
50 milligrams of HEMOXCell® are sufficient to perform 2 x 96-Well plate cultures.
FLASK
262.5 mg of HEMOXCell® are sufficient to perform in four 500 ml flasks with three different concentrations (0.025, 0.05, and 0.1)
BIOREACTOR
Bioreactor: 2.5 g of HEMOXCell® are sufficient to prepare 100L media at 0.025 mg/ml
COMPOSITION
Natural extracellular hemoglobin Buffer: Trizma base (Sigma aldrich)
CONCENTRATION RANGE
Concentration in hemoglobin is 50 mg - 5g ±5 mg/mL
OSMOLARITY
303 mOsm/kg ± 10
MICROBIOLOGICAL CHARACTERISTICS
• Aseptic manufacturing process
• Microbial contamination free
• Sterility guaranteed
ACADEMIC DISCOUNT
Available
PROPERTIES
• Biopolymer of 3.6 MDa
• Structure: 156 oxygen carrier chains and 42 linker chains
• Antioxidative properties (SOD activity)
• Loading and unloading oxygen in PO2 gradient
• Origin: Nereis virens (marine invertebrate) in-vivo synthesis and biotechnology process based on an extracted and purified compound
• The active substance is suspended in a stabilizing buffer
RISK IDENTIFICATION
Extracted from a non-GMO biological product (Nereis virens), it does not contain any component dangerous to human health. Proteins contained in aqueous media.
COMPOSITION OF INGREDIENTS
Extracellular hemoglobin extracted from marine invertebrate Nereis virens. No dangerous components at all (obtained by extraction of natural material)
FLAMMABILITY EXTINCTION
No flammable product
RISK OF EXPLOSION
No danger if there is decomposition
COMBUSTION
No danger of combustion
STABILITY
Stable product in storage. No dangerous reaction in normal conditions or use. No dangerous decomposition products. Polymerization possible if combined with substances like a reticulation agent
NATURAL BIOLOGICAL PRODUCT
Obtained by extraction from Nereis virens
PRODUCT DISPOSAL
Animal product, Follow local regulations for disposal
SOILED PACKAGING
Disposal of soiled packaging must follow local regulations
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.