Description
The SensoLyte® Plus 520 MMP-1 Assay Kit is designed for specifically detecting MMP-1 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-1 monoclonal antibody is used in combination with an MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-1-induced cleavage of the FRET substrate.
The SensoLyte® Plus 520 MMP-1 Assay Kit is designed for specifically detecting MMP-1 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-1 monoclonal antibody is used in combination with an MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-1-induced cleavage of the FRET substrate.
Specifications
COUNTRY OF ORIGIN
United States of America
APPLICATION
ELISA + FRET
Protease activity
*USAGE / SAFETY STATEMENT
This product is for research or laboratory use only and are not to be administrated to humans. This product is not intended for medical diagnostic or therapeutic use.
DISCLAIMER
Labscoop LLC and/or Kaneka Eurogentec S.A.'s liability shall be limited strictly to the replacement of non-compliant products or to the reimbursement of their price, at Labscoop LLC and/or Kaneka Eurogentec S.A.'s sole election. To the full extent permissible under the applicable legislation Labscoop LLC nor Kaneka Eurogentec S.A. may not be held liable for any cost or liability arising from or in connection with this product, including damages or accidents to persons, damages to goods other than this product sold, loss of earnings or profits, harm to reputation, or any other prejudice arising directly or indirectly from the product, and including defective products. IN NO EVENT SHALL LABSCOOP LLC OR KANEKA EUROGENTEC S.A. BE LIABLE UNDER THIS AGREEMENT FOR ANY PUNITIVE, EXEMPLARY, INDIRECT OR CONSEQUENTIAL DAMAGES, INCLUDING LOST PROFITS. This product ordered by the customer are exclusively intended for laboratory research purposes and shall correspond to the quality and safety standards in accordance with said research purposes. This product shall not be used by the customer for any other purposes such as (the list is not exhaustive) diagnostic, prophylactic, therapeutic, cosmetic, commercial ends, or as food, ingredients or medical devices. Labscoop LLC and Kaneka Eurogentec S.A. shall accept no liability if the customer who ordered this product, intended exclusively for laboratory research, uses this product for purposes other than for research.
QUALITY CERTIFICATION / COMPLIANCE
Eurogentec Quality Management System is ISO 9001 certified by BSI under certificate number FS 638601. The certification applies to following activities: “Development, production and sales of products and services for research and development in life sciences”. Regulations for in vitro diagnostics (IVD) - Eurogentec manufactures products in support of in vitro diagnostic and related applications. The IVD medical device industry is regulated by FDA GMP/QSR (21 CFR Part 820), EU IVD Directive 98/79 EEC and EU 2017/746 Regulation. These regulations are not applicable to Eurogentec as it does not manufacture medical devices. However Eurogentec quality system is compliant to the applicable quality system requirements of these regulations.
DETECTION METHOD
Fluorescent
KIT COMPONENTS
Component A: Microplate coated with monoclonal anti human MMP-1 antibody: 12 X 8 black strips Component B: MMP-1 standard, recombinant human proMMP-1: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 10 mL Component D: 10 X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contains organic mercury. Dispose according to regulations): 100 mM, 150 µL Component F: MMP-1 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Assay buffer: 50 mL Component H: Stop Solution: 10 mL Component I: Adhesive cover strip: 3 sheets
RESEARCH AREA
Adhesion & Extracellular Matrix
Cancer & Apoptosis
Peptide & Protein Analysis
BIOMARKER TARGET
Matrix Metalloproteinase (MMP)
MMP-1