For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
COUNTRY OF ORIGIN
China
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
MWCO (kDa)
55kDa
SOURCE
Rabbit
ANTIBODY TYPE
Polyclonal antibody
ISOTYPE
Rabbit IgG
PURIFICATION
Affinity purified
IMMUNOGEN
Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).
PRESERVATIVE
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SWISS-PROT NO.
Q969G3
ALTERNAME
SMARCE1
CELLULAR LOCALIZATION
Nucleus
RELEVANCE
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth . Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells.
RECOMMENDED DILUTIONS
WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200(Optimal dilutions should be determined by the end user)
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store at -20°C. Avoid freeze / thaw cycles.
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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