TUNEL in situ cell apoptosis detection kit, cell

China

For laboratory research and development purposes only.

TUNEL Apoptosis Detection Kit for adherent cells (Biotin labeled POD) is one of EnoGene’s newly introduced products. It is used for detecting the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase, (TdT or rTdT). Horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide and 3, 3’-Diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.