Anti-PD-1 (RMP1-14) 5mg/ml

United Kingdom

RMP1-14

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

>95% by SDS-PAGE and HPLC

5mg/ml

Western Blot, Blocking, Flow Cytometry, Functional Assays

Rat

Rat IgG2a Kappa

Mouse

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Mouse PD-1 transfected BHK cells

Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications.

Subset of double negative thymocytes, activated T and B cells

Aggregation level ≤ 5%

≤ 1.0 EU/mg as determined by the LAL method

PD-1