Specifications
COUNTRY OF ORIGIN
United Kingdom
*USAGE / SAFETY STATEMENT
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
PURITY
>95% by SDS-PAGE and HPLC
APPLICATION.1
IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, CyTOF
PURIFICATION
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
FORMULATION
0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added.
SPECIFICITY
Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16
STORAGE BUFFER
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
APPLICATION NOTES
Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume.
Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation.
Each investigator should determine their own optimal working dilution for specific applications.
Antigen Distribution
CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.
Aggregation
Aggregation level ≤ 5%
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
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