Specifications
COUNTRY OF ORIGIN
United Kingdom
*USAGE / SAFETY STATEMENT
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
PURITY
>95% by SDS-PAGE and analyzed by silver stain.
CONCENTRATION
1.0 - 5.0 mg/ml
APPLICATION.1
Flow Cytometry, Western Blot, IHC (Frozen), Immunofluorescence, Immunoprecipitation
PURIFICATION
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
FORMULATION
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
SPECIFICITY
Anti-CD14 In Vivo Antibody - Low Endotoxin (UCHM-1) recognizes Human CD14. Anti-Human CD14 recognizes a (Mr 55 kDa) cell surface glycoprotein expressed on most peripheral blood monocytes.
STORAGE BUFFER
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
APPLICATION NOTES
Each investigator should determine their own optimal working dilution for specific applications.
Antigen Distribution
The CD14 antigen is expressed on approximately 70-95% of human peripheral blood monocytes. It is also found on other cells of myeloid lineage including granulocytes, dendritic reticulum cells and pleural phagocytic cells.
Aggregation
Aggregation level ≤ 5%
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
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