CSC 2F0 cells were isolated by centrifugal counterflow elutriation from 250 individual donor foreskins and are available in single, cryopreserved vials or proliferating flasks. Cell Systems primary cells are unadulterated, healthy cells with a high yield that are ideal for 2D or 3D cell cultures. Use of Cell Systems Media and Reagents is highly recommended.
Primary cells are available at Passage 3 [<12 cumulative population doublings] cryopreserved in Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75 cm2 flask. The cells were originated using Cell Systems...
CSC 2F0 cells were isolated by centrifugal counterflow elutriation from 250 individual donor foreskins and are available in single, cryopreserved vials or proliferating flasks. Cell Systems primary cells are unadulterated, healthy cells with a high yield that are ideal for 2D or 3D cell cultures. Use of Cell Systems Media and Reagents is highly recommended.
Primary cells are available at Passage 3 [<12 cumulative population doublings] cryopreserved in Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75 cm2 flask. The cells were originated using Cell Systems...
Shipping & Handling
Shipped In
Dry Ice
Safety & Storage
Storage Temperature
Remove the flask from the shipping pouch. Do not remove the seal or the Transport Medium. Place the flask in a 37ºC incubator. Stand the flask on its end to expose maximum surface area to the warm, humidified, circulating atmosphere. Do not place the flask flat. The steel shelf will give up heat to the cell-bearing bottom surface of the flask much too fast, sandwiching the cells between a heat source (the shelf) and a heat sink (the Transport Medium). This is too much ∆T (change in temperature) for the cells to accommodate without significant damage to the cytoskeleton. Two to three hours later, during which time the feeding medium has been warmed to 37ºC in a water bath, aspirate (and discard) the Transport Medium and feed with 5-7 ml Complete Classic Medium (4Z0-500) supplemented with CultureBoost™. Transport Medium is neither intended nor suitable for maintaining cells in the incubator.
Safety Statement
For laboratory research and development purposes only.
For laboratory research and development purposes only.
SIZE
Frozen Vial with 1E6 cells/1mL
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Dry Ice
Safety & Storage
Storage Temperature
Remove the flask from the shipping pouch. Do not remove the seal or the Transport Medium. Place the flask in a 37ºC incubator. Stand the flask on its end to expose maximum surface area to the warm, humidified, circulating atmosphere. Do not place the flask flat. The steel shelf will give up heat to the cell-bearing bottom surface of the flask much too fast, sandwiching the cells between a heat source (the shelf) and a heat sink (the Transport Medium). This is too much ∆T (change in temperature) for the cells to accommodate without significant damage to the cytoskeleton. Two to three hours later, during which time the feeding medium has been warmed to 37ºC in a water bath, aspirate (and discard) the Transport Medium and feed with 5-7 ml Complete Classic Medium (4Z0-500) supplemented with CultureBoost™. Transport Medium is neither intended nor suitable for maintaining cells in the incubator.
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.