ANXA1 active protein (prokaryotic), Human

Cloud-Clone Corp. Wuhan

SKU / CAT#: APE787Hu01-10ug

^$228⁰⁰

usd

^$22.80/ug

APE787Hu01-10ug

ANXA1 active protein (prokaryotic), Human

Name: ANXA1 active protein (prokaryotic), Human
Price: 228.00 USD
Availability: InStock

SKU / CAT#: APE787Hu01-10ug

MPN:

^$228⁰⁰

usd

^$22.80/ug

Available

SHIPS Jul 9th

Available

SHIPS Jul 9th

SKU

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Description

ANXA1 active protein (prokaryotic), Human

Shipping & Handling

Shipped In

Cold packs

Safety & Storage

Storage Temperature

Store at 2-8℃ for one month,-80℃ for 12 months

Safety Statement

Research Use Only

Regulatory & Compliance

Technical Documents

MANUAL (1)

Product Manual for ANXA1 active protein (prokaryotic), Human

Specifications

CLASS

Active proteins

SHIPPING NOTES

FedEx Service

COUNTRY OF ORIGIN

China

RAW MATERIAL ORIGINS

China

APPLICATION

Cell culture; Activity Assays.

USAGE

Reconstitute in PBS or others.

GENE ID

https://www.ncbi.nlm.nih.gov/gene/301

UNIPROT ID

P04083

*USAGE / SAFETY STATEMENT

Research Use Only

PURITY

Greater than 97% by SDSPAGE

pH RANGE

7.4

CONCENTRATION

0.2mg/mL

MWCO (kDa)

44kDa

VALIDATED APPLICATION

Cell culture; Activity Assays.

SOURCE

E.coli

HOST SPECIES

E.coli

REACTIVITY SPECIES

Human

CROSS REACTIVITY

Human

Questions & Answers (0)

Citations (0)

Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2 -dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. Besides, S100 Calcium Binding Protein A11 (S100A11) has been identified as an interactor of ANXA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ANXA1 and recombinant human S100A11. Briefly, ANXA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to S100A11-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ANXA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ANXA1 and S100A11 was shown in Figure 1, and this effect was in a dose dependent manner.<br/>Figure. The binding activity of ANXA1 with S100A11.

Shipping & Handling

Shipped In

Cold packs

Safety & Storage

Storage Temperature

Store at 2-8℃ for one month,-80℃ for 12 months

Safety Statement

Research Use Only

Regulatory & Compliance

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