Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and...
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and...
Shipping & Handling
Shipped In
Blue Ice
Safety & Storage
Storage Temperature
Store the ACTA2 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Safety Statement
For research use only.
Regulatory & Compliance
Specifications
CLASS
Primary Antibody
APPLICATION
IF, IHC-P
*USAGE / SAFETY STATEMENT
For research use only.
PURITY
Protein G affinity chromatography
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human, Rat
CLONALITY
Clonality.MONOCLONAL
CONJUGATION/TAG
Unconjugated
APPLICATION DILUTIONS
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the ACTA2 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
CUSTOM CONJUGATIONS AVAILABLE
No
CLONE
1A4 + ACTA2/791
PRESERVATIVE.1
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Blue Ice
Safety & Storage
Storage Temperature
Store the ACTA2 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Safety Statement
For research use only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.