This is a subclone of ‘L’ cells originally established by W R Earle in 1940. It was derived from normal subcutaneous tissue of a 100-day old male C3H/An mouse.
This is a subclone of ‘L’ cells originally established by W R Earle in 1940. It was derived from normal subcutaneous tissue of a 100-day old male C3H/An mouse.
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
Store frozen vials in liquid nitrogen at -196°C. Do not store at -80°C freezer.
Safety Statement
For research use only.
Regulatory & Compliance
Specifications
APPLICATION
RNA-I, WB
*USAGE / SAFETY STATEMENT
For research use only.
PHYSICAL FORM
Provided as a frozen vial in our freeze media or as actively growing culture in a T25 flask.
APPLICATION NOTES
Uses: For virus titration, generation of recombinant cell lines and protein production. For laboratory reseh use only. Type: Mouse connective tissue established cell line. Growth Media: DMEM with 5%-10% Fetal Calf Serum. Incubate in 5% CO2; at 37°C. Morphology: Fibroblasts. Growth Mode: Adherent monolayer. Subculture: Split culture using 0.25% Trypsin when about 80% confluent and re-seed at about 4000 cells per cm2
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Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
Store frozen vials in liquid nitrogen at -196°C. Do not store at -80°C freezer.
Safety Statement
For research use only.
Regulatory & Compliance
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