Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).
COUNTRY OF ORIGIN
China
TARGET ORGANS
Cancer Antigen CA19-9
*USAGE / SAFETY STATEMENT
This product is for research use only. It is not intended for diagnostic or therapeutic use.
REACTIVITY SPECIES
Human
DETECTION METHOD
Colorimetric
SENSITIVITY
0.5 U/ml
PRODUCT RANGE
3.12-200 U/ml
SAMPLE TYPE
Serum, plasma, and cell culture supernatants.
PRINCIPLE ASSAY
This Cancer Antigen CA19-9 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Cancer Antigen CA19-9 Antibody. Standards or samples are then added to the microtire plate wells and Cancer Antigen CA19-9, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Cancer Antigen CA19-9 present in the sample, Anti-Cancer Antigen CA19-9 Antibody (Biotin) is added to each well to "sandwich" the Cancer Antigen CA19-9 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Cancer Antigen CA19-9 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
ASSAY TYPE
Sandwich (quantitative)
PLATFORM
Microplate
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store at 2-8°C for 4 months or at -20°C for 8 months.
Safety Statement
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Regulatory & Compliance
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