Anti-FOXP3 Antibody

Primary

Polyclonal

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

United States of America

ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB

FOXP3

This product is for research use only. It is not intended for diagnostic or therapeutic use.

0.7 µg/µl in antibody stabilization buffer

Rabbit

Bovine, Feline, Human, Monkey, Mouse, Rat

Polyclonal

Unconjugated

ELISA: 1:15,000-1:20,000; ELISA: 1:15,000-1:20,000; Immunocytochemistry: 1:100; Immunofluorescence: 1:100; Immunohistochemistry: 1:100; Immunoprecipitation: 1:200; Western Blot: 1:500

Synthetic peptide corresponding to amino acids 229-250 of Mouse Forkhead Box Protein 3 (winged helix transcription factor 3 protein). The peptide was post synthetically modified to achieve desired antigenicity before coupling to a carrier protein.

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells ( Treg ). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed :15790681 ). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed :17360565 ). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed :17377532 ). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed :18354202 ). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.