JOE phosphoramidite, 6-isomer

JOE phosphoramidite, 6-isomer. JOE phosphoramidite for oligonucleotide synthesis, pure 6-isomer (6-JOE). Fluorescent dye JOE is a fluorescein derivative containing two chlorine atoms and two methoxy groups. Its absorption and emission maxima are at 503 nm and 525 nm, respectively. By its spectral characteristics JOE is found in between FAM and TAMRA/ROX; therefore, this fluorophor is commonly used for multiplex detection, including that during DNA sequencing. Coupling time of 6 minutes. Deprotection can be perfromed under standard conditions using ammonium hydroxide; deprotection time depends...