Anti-p53 Antibody [TP53/2092R] - BSA and Azide free

Primary

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

This product is for research use only. It is not intended for diagnostic or therapeutic use.

WB, IHC-P

1 mg/ml

Liquid

Monoclonal

TP53/2092R

Rabbit

IgG

Human, Monkey, Canine, Hamster, Chicken

Monoclonal

Protein A chromatography.

Recombinant full length human TP53 protein.

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Unconjugated

This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

p53

This antibody does not cross react with Mouse or Rat.

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