Human LMAN2 ELISA Kit

Sandwich (quantitative)

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

United States of America

Sandwich ELISA

LMAN2

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Human

This assay has shown no significant cross-reactivity.

Colorimetric

7.26 pg/ml

This kit can assay both natural and recombinant human LMAN2.

62.50 - 4000 pg/ml

LMAN2 (Lectin mannose-binding 2), also known as VIP36, is a protein that in humans is encoded by the LMAN2 gene. LMAN2 has been originally isolated from MDCK cells as a component of glycolipid-enriched detergent-insoluble complexes containing apical marker proteins. LMAN2 binds glycoproteins that retain the intact D1 mannosyl branch in the cis-Golgi network and recycles to the endoplasmic reticulum where, due to higher pH, it releases its cargos, thereby contributing to the quality control of glycoproteins. LMAN2 is an intracellular lectin that cycles between the endoplasmic reticulum and the Golgi apparatus, and is thought to act as a cargo receptor in the transport and sorting of glycoproteins. The interaction between recombinant soluble LMAN2 and BiP is dependent on divalent cations but not on ATP.

Cell culture supernatant, serum and plasma.

Human LMAN2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human LMAN2 in the samples. A monoclonal antibody specific for human LMAN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for LMAN2 is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of LMAN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Microplate

84% (Average Recovery from Serum Samples).