Human IL 23 ELISA Kit

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

United States of America

Sandwich ELISA

IL 23

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Human

This assay has shown no significant cross-reactivity.

Colorimetric

1.82 pg/ml

This kit can assay both natural and recombinant human IL-23.

31.25 - 2000 pg/ml

Interleukin 23 (IL-23) is a heterodimeric cytokine that is related to IL-12. IL-23 is a heterodimeric cytokine composed of an IL-12p40 subunit that is shared with IL-12 and the IL-23p19 subunit. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12Rβ1 and IL-23R. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naïve and memory T cells, as compared to IL-12. IL-23 effects on IL-17-producing T cells may also enhance the development of several models of autoimmune disease including experimental allergic encephalomyelitis (EAE), collagen-induced arthritis (CIA), colitis, and diabetes. IL-23 may also play a role in increased tumor growth associated with chronic inflammation. In humans, IL-23 has been found upregulated in several pathologies with dysregulated immune function including psoriasis, Crohn’s disease, and multiple sclerosis.

Cell culture supernatant, serum and plasma.

Human IL-23 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IL-23 in the samples. A monoclonal antibody specific for human IL-23 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-23, and IL-23 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-23 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Sandwich (quantitative)

Microplate

99% (Average Recovery from Serum Samples).