SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells.
Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes.
Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes.
Optimal dilutions to be determined...
SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells.
Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes.
Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes.
Optimal dilutions to be determined...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Adapter Proteins
APPLICATION
IC IP WB
CLONE NUMBER
5H43 (84)
ACCESSION#
NM_031892
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
rat
ISOTYPE
IgG1,k
CROSS REACTIVITY
Hu Mo Rt
IMMUNOGEN
6His and HA tagged fusion protein corresponding to full length rat SETA.
SPECIFIC ACTIVITY
Recognizes rat SETA, Mr ~85kD. SETA has multiple splice variants which can result in multiple bands in Western Blot. Species Crossreactivity: human and mouse.
SWISS PROT#
Q96B97
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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