Applications:
Suitable for use in Immunofluorescence, Flow Cytometry (Not Tested), Immunoblot, Methylated DNA Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry (Not Tested): 10ul labels 10e6 cells in 100ul
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
Store product at 4°C if to be used immediately within...
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry (Not Tested), Immunoblot, Methylated DNA Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry (Not Tested): 10ul labels 10e6 cells in 100ul
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
Store product at 4°C if to be used immediately within...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Nucleotides
APPLICATION
IF IHC IP WB
CLONE NUMBER
10B11 (33D3)
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein A affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG1
CROSS REACTIVITY
Hu Mo
IMMUNOGEN
5-methylcytosine conjugated to Ovalbumin
SPECIFIC ACTIVITY
Recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. Specific to the methylated base and has
minimal reactivity to non-methylated cytidine or cytosine. Species Crossreactivity: human and mouse.
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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