Pico Methyl-Seq Library Prep Kit

It was relatively easy to use and troubleshoot. Some of the master mixes did run out quickly though, so more kits had to be purchased to accommodate the missing volume.

It is an easy to use product with very simple and detailed protocol. Usually library preps are an over-complicated process, especially in case of DNA methylation analysis. It has the advantage of generating a library from very small amount of DNA. It also includes all the required primers for sequencing, although it needs to be updated to support dual barcoding. With most problematic samples the mapping efficiency obtained using this kit is a bit concerning because it might fall below 30%, it can be improved though.

This works for non-model organisms such as corals. However, we had to make a few modifications which included increasing the volume of the library amp master mix. Unfortunately, you cannot purchase this separately at the moment, so the only way to get more of this master mix is by purchasing an entire kit (which is very expensive!).