The RRHP 5-hmC Library Prep Kit is an all-inclusive solution for analysis of genome-wide 5-hydroxymethylcytosine (5-hmC) positions at single-base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Fragments lacking glucosylated 5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments containing 5-hmC will be successfully amplified and analyzed by Next-Generation Sequencing. Fragments with higher 5-hmC levels will be correlated...
The RRHP 5-hmC Library Prep Kit is an all-inclusive solution for analysis of genome-wide 5-hydroxymethylcytosine (5-hmC) positions at single-base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Fragments lacking glucosylated 5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments containing 5-hmC will be successfully amplified and analyzed by Next-Generation Sequencing. Fragments with higher 5-hmC levels will be correlated...
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See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
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COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
SOURCE
High quality, intact genomic DNA free of enzymatic inhibitors.
INPUT
100 ng - 1 µg
KIT COMPONENTS
● dNTP Mix [10 mM] (D1000-1). _x000D_
EQUIPMENT
Microcentrifuge, thermocycler with heated lid, and gel electrophoresis or other equipment for DNA visualization
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Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
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