NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling]

HPLC Derivatization Reagents

Japan

For laboratory research and development purposes only.

>98.0%(HPLC)

HPLC Labeling Reagent for Carbonyl Compounds The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carbonyl group to form the corresponding hydrazone. The labeling reagent itself is non-fluorescent, but the hydrazones after the reaction with carbonyl compounds have strong fluorescence. The resultant hydrazone is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 550 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants, and a highly sensitive detection can be done because of its high reactivity. Application examples: [Aldehydes or ketones] 1) 250 µM of the labeling reagent 1 and 1.7 µM propionaldehyde are added to acetonitrile containing 0.025% TFA, and reacted at room temperature for 1 h, use it as the HPLC sample. For example, the detection limit for propionaldehyde is 35 fmol. TCI Products Pamphlet HPLC Labeling Reagents ( PDF 10MB )

C6H5N5O3·H4N2

Solid

227.18

Fukaya factory is certified for GMP, ISO14001:2004 (JQA-EM5386) and IQ Net (JP-JQA-EM5386).

P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P302 + P352 : IF ON SKIN: Wash with plenty of soap and water. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P362 : Take off contaminated clothing and wash before reuse.

Fluorescence Detection Labeling Reagents (for Carbonyl Groups) [HPLC Derivatization Reagents]

MFCD00191508

GHS07

Orange to Brown powder to crystal

87563175.0

Warning

H315 : Causes skin irritation. H319 : Causes serious eye irritation.

2934.99.4400

>98.0%(HPLC)

min. 98.0 area%