Detecting Epigenetic DNA Methylation in Arabidopsis Amplification and Electrophoresis Kit with GelGreen® Stain (with perishables)

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For Educational & Research Use Only (RUO). Not for diagnostic purposes.

1 Arabidopsis Seed Pack from Ler (Landsberg erecta) Wild Type  1 Arabidopsis Seed Pack from fwa-1 (flowering wageningen) Mutant  1 FWA Primer/Loading Dye Mix, 700 µL  1 McrBc Buffer, 10x, 200 µL  1 McrBc Enzyme, 10 U/µL, 20 µL  1 Guanosine 5′–Triphosphate (GTP), 100 mM, 15 µL  1 BSA, 10 mg/mL, 30 µL  1 Sterile Distilled Water, 1 mL  1 RNAse Solution, 5 mg/mL, 250 µL  1 Potting Soil, Bag  1 pBR322/BstNI Markers, 0.075 µg/µL, 130 µL  25 Ready-to-Go™ PCR Beads, 0.2-mL Tubes  1 Nuclei Lysis Solution, 12 mL 1 Protein Precipitation Solution, 3.5 mL 2 Isopropanol, 100%, 20 mL 1 Ethanol, 70%, 30 mL 1 DNA Rehydration Buffer, 2.5 mL 15 Plastic Pestles 1 Mineral Oil, 5 mL 2 Agarose, 4 g 1 20x TBE Buffer, 150 mL 1 GelGreen® Nucleic Acid Gel Stain, 100 µL 1 NaCl, 5 M, 8 mL 12 Latex Gloves 6 Staining Trays 1 Planting Flat and Tray 1 Plastic Dome Lid 1 Teachers Manual with Reproducible Student Guide ,Tweezers, Scissors, or Scalpel  Water Baths/Heating Blocks (65° C and 37° C)  Micropipets and Tips, 1 to 1,000 µL  Microcentrifuge 32 Microcentrifuge Tubes, 1.5 mL 8 Microcentrifuge Tube Racks 1 Thermal Cycler, Programmable  Electrophoresis Chambers  Electrophoresis Power Supplies  Container with Ice  Computer with Internet Access  Microwave, Hot Plate, or Boiling Water  Vortexer (optional)