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PTC Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® and 0.2-mL Tubes (with prepaid coupon)
Carolina Biological Supply Company
SKU / CAT#: 211381
$25200
usd
$252.00/Kit
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  4. PTC Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® and 0.2-mL Tubes (with prepaid coupon)
211381

PTC Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® and 0.2-mL Tubes (with prepaid coupon)

Carolina Biological Supply Company
1review
1
0citationscitations
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0questionsquestions
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SKU / CAT#: 211381
MPN: 211381
$25200
usd
$252.00/Kit
Available
SHIPS Jun 16th
Available
SHIPS Jun 16th
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Description
Advanced - For experienced high school and college classes; requires some basic technical skill. This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC), which was first described in the 1930s by Cold Spring Harbor Laboratory scientist Albert Blakeslee. Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene. The 2 alleles differ by a single nucleotide, so restriction...
Advanced - For experienced high school and college classes; requires some basic technical skill. This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC), which was first described in the 1930s by Cold Spring Harbor Laboratory scientist Albert Blakeslee. Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene. The 2 alleles differ by a single nucleotide, so restriction...
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product insert and individual kit component labels for more information.
Safety Statement
For Educational & Research Use Only (RUO). Not for diagnostic purposes.
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Specifications
CLASS
PCR
*USAGE / SAFETY STATEMENT
For Educational & Research Use Only (RUO). Not for diagnostic purposes.
KIT COMPONENTS
1 pBR322/BstNI Markers, 0.075 μg/μL, 200 μL 1 HaeIII Restriction Enzyme, 40 μL 1 PTC Primer/Loading Dye Mix, 700 μL 1 Chelex® Resin, 1.5 g 25 Ready-to-Go™ PCR Beads (0.2 mL tubes) 1 Mineral Oil, 5 mL 100 PTC Taste Strips 100 Control Taste Strips 1 Agarose, 10 g 1 TBE Buffer, 200 mL 1 CarolinaBLU® Final Stain, 375 mL 1 CarolinaBLU® Gel and Buffer Stain, 7 mL 12 Latex Gloves 9 Staining Trays 1 Teachers Manual and Reproducible Student Guide,Saline Solution, 0.9%  Micropipets and Tips, 1 μL to 1000 μL  Microcentrifuge Tubes, 1.5 mL  Microcentrifuge Tube Racks  Microcentrifuge for 1.5 mL Tubes  PCR Tubes, 0.2 or 0.5 mL  Tube Adapters for Microcentrifuge, 0.2 or 0.5 mL  Programmable Thermal Cycler  Electrophoresis Chambers  Electrophoresis Power Supplies  Gel-staining Trays  White Light Box  Paper Cups  Permanent Markers  Contrainer with Cracked or Crushed Ice  Boiling Water Bath (optional)  Camera (optional)
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Reviews & Ratings (1)
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An independent study student did this as a project with me. It was easy to use, and all but one student gave clear, easily-interpretable results. She had tried...
Dennis
NORTH CAROLINA
An independent study student did this as a project with me. It was easy to use, and all but one student gave clear, easily-interpretable results. She had tried...
Dennis
NORTH CAROLINA
Invalid date
Straight forward and works really well!
Dennis C.
NORTH CAROLINA
An independent study student did this as a project with me. It was easy to use, and all but one student gave clear, easily-interpretable results. She had tried a "bio-hacking" kit from a different manufacturer that had a PTC molecular genotype component, and it was a nightmare. It just did not work, and we tried it three times. Then the Carolina kit worked perfectly the first time! Go Carolina!
Citations (0)
PTC Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® and 0.2-mL Tubes (with prepaid coupon)
PTC Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® and 0.2-mL Tubes (with prepaid coupon)
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product insert and individual kit component labels for more information.
Safety Statement
For Educational & Research Use Only (RUO). Not for diagnostic purposes.
Regulatory & Compliance
Badge
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