KIT COMPONENTS
1 PV92B Primer/Loading Dye Mix, 700 µL 1 pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL 1 Proteinase K, 100 µg/mL, 5 mL 1 Chelex® Resin, 0.5 g 25 PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity) 1 Mineral Oil, 5 mL 1 Agarose, 5 g 1 TBE, 150 mL 1 Ethidium Bromide, 1 µg/mL, 250 mL 4 Latex Gloves 6 Staining Trays 1 Alu CD-ROM 1 Teachers Manual with Reproducible Student Guide,Saline Solution, 0.9% (10 mL per student) Micropipets and Tips (1 µL to 1,000 µL) Microcentrifuge Tubes, 1.5 mL (2 per student) Microcentrifuge Tube Racks Microcentrifuge for 1.5-mL Tubes PCR Tubes, 0.2 mL or 0.5 mL (1 per student) Tube Adapters (for microcentrifuge) Thermal Cycler Electrophoresis Chambers Electrophoresis Power Supplies Gel Staining Trays UV Transilluminator (for ethidium bromide stain) Paper Cup (1 per student) Permanent Markers Container of Crushed Ice Camera (optional) Boiling Water Bath (optional)