KIT COMPONENTS
1 E. coli Culture 1 pBLU® Plasmid Solution, 200 µL 1 Ampicillin, 4 mL 1 Ampicillin/X-gal Solution, 2% in DMF, 5.4 mL 3 Sterile LB Agar, 400 mL 50 Sterile Petri Dishes 8 50 mM Calcium Chloride, 3 mL 8 LB Broth, 3 mL 16 Transformation Tubes, 15 mL 48 Sterile Transfer Pipets, 1 mL 1 Glass Beads, 1 oz 28 Sterile Transfer Loops 1 Wire Inoculating Loop 1 Teachers Manual and Reproducible Student Guide,1 Boiling Water Bath or Microwave (for melting agar) 8 Beakers, 600 mL 1 Container (for glass beads) 8 Culture Tube Racks 8 Wax Markers 1 Masking Tape 1 Bunsen Burner 1 Water Bath, 42°C 1 Autoclavable Disposable Bag Ice Parafilm® or Plastic Wrap Bleach Solution, 10% Incubator (optional)
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