The calpains are a family of intracellular Ca2+ dependent cysteine proteases. The best-characterized calpains are isoforms 1 (µ) and 2 (m). They respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). Calpains are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer’s disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.
The SensoLyte® 520 Calpain Assay Kit is optimized...
The calpains are a family of intracellular Ca2+ dependent cysteine proteases. The best-characterized calpains are isoforms 1 (µ) and 2 (m). They respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). Calpains are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer’s disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.
The SensoLyte® 520 Calpain Assay Kit is optimized...
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Safety & Storage
Storage Temperature
Store component D at -80°C. Store all other components at -20°C. Component C can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Safety Statement
This product is for research or laboratory use only and are not to be administrated to humans. This product is not intended for medical diagnostic or therapeutic use.
This product is for research or laboratory use only and are not to be administrated to humans. This product is not intended for medical diagnostic or therapeutic use.
KIT COMPONENTS
Component A: 5-FAM/QXL 520 Calpain Substrate, Ex/Em=490 nm/520 nm upon cleavage: 1 mM, 50 µL Component B: 5-FAM, Fluorescence Reference Standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Assay Buffer: 20 mL Component D: Human Calpain: 0.5 mg/mL, 100 µL Component E: Calpain Inhibitor: 50 µM, 10 µL Component F: DTT: 1 M, 20 µL
APPLICATION
FRET
Protease activity
DISCLAIMER
Labscoop LLC and/or Kaneka Eurogentec S.A.'s liability shall be limited strictly to the replacement of non-compliant products or to the reimbursement of their price, at Labscoop LLC and/or Kaneka Eurogentec S.A.'s sole election. To the full extent permissible under the applicable legislation Labscoop LLC nor Kaneka Eurogentec S.A. may not be held liable for any cost or liability arising from or in connection with this product, including damages or accidents to persons, damages to goods other than this product sold, loss of earnings or profits, harm to reputation, or any other prejudice arising directly or indirectly from the product, and including defective products. IN NO EVENT SHALL LABSCOOP LLC OR KANEKA EUROGENTEC S.A. BE LIABLE UNDER THIS AGREEMENT FOR ANY PUNITIVE, EXEMPLARY, INDIRECT OR CONSEQUENTIAL DAMAGES, INCLUDING LOST PROFITS. This product ordered by the customer are exclusively intended for laboratory research purposes and shall correspond to the quality and safety standards in accordance with said research purposes. This product shall not be used by the customer for any other purposes such as (the list is not exhaustive) diagnostic, prophylactic, therapeutic, cosmetic, commercial ends, or as food, ingredients or medical devices. Labscoop LLC and Kaneka Eurogentec S.A. shall accept no liability if the customer who ordered this product, intended exclusively for laboratory research, uses this product for purposes other than for research.
QUALITY CERTIFICATION / COMPLIANCE
Eurogentec Quality Management System is ISO 9001 certified by BSI under certificate number FS 638601. The certification applies to following activities: “Development, production and sales of products and services for research and development in life sciences”. Regulations for in vitro diagnostics (IVD) - Eurogentec manufactures products in support of in vitro diagnostic and related applications. The IVD medical device industry is regulated by FDA GMP/QSR (21 CFR Part 820), EU IVD Directive 98/79 EEC and EU 2017/746 Regulation. These regulations are not applicable to Eurogentec as it does not manufacture medical devices. However Eurogentec quality system is compliant to the applicable quality system requirements of these regulations.
Cancer & Apoptosis
Cardiovascular Research
Cell Signaling
Diabetes
Neuroscience
Peptide & Protein Analysis
DETECTION METHOD
Fluorescent
BIOMARKER TARGET
Calpain
DETECTION LIMIT
32.5 ng/ml
NACRES
NA.32
ABSORBANCE
490
EMISSIONS
520
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store component D at -80°C. Store all other components at -20°C. Component C can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Safety Statement
This product is for research or laboratory use only and are not to be administrated to humans. This product is not intended for medical diagnostic or therapeutic use.
Regulatory & Compliance
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