Mps-1 is a protein kinase thought to play during the spindle checkpoint to regulation of sister chromatids separation when chromsome alingment is complete.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.5-2ug/ml of this lot detected Mps1 in HeLa total cell extract. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with M4685-80 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation:...
Mps-1 is a protein kinase thought to play during the spindle checkpoint to regulation of sister chromatids separation when chromsome alingment is complete.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.5-2ug/ml of this lot detected Mps1 in HeLa total cell extract. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with M4685-80 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation:...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Protein Kinases
APPLICATION
IC IP WB
CLONE NUMBER
5H9 (4-112-3)
ACCESSION#
NM_003318
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG1
CROSS REACTIVITY
Hu
IMMUNOGEN
His-tagged fusion protein corresponding to aa3-856 of human Mps1, previously known at TTK/PYK.
SPECIFIC ACTIVITY
Recognizes human Mps1, Mr ~97kD. This antibody is known to inhibit the autophosphorylation/ kinase activity of Mps1. Does not react with mouse.
SWISS PROT#
P33981
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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