Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: detected LKB1 in RIPA lysates from Rat2 cells. Rat2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with L3185-03 (1ug/ml). Proteins were visualized using a IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunocytochemistry: detected LKB1 in transfected Cos-7 cells fixed with 3-7% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Optimal dilutions to be determined by the researcher.
*Recommended...
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: detected LKB1 in RIPA lysates from Rat2 cells. Rat2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with L3185-03 (1ug/ml). Proteins were visualized using a IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunocytochemistry: detected LKB1 in transfected Cos-7 cells fixed with 3-7% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Optimal dilutions to be determined by the researcher.
*Recommended...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Protein Kinases
APPLICATION
IC WB
CLONE NUMBER
5H64
ACCESSION#
NM_000455
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG
CROSS REACTIVITY
Hu Mo Rt
IMMUNOGEN
GST fusion protein corresponding to residues 1-433 of human LKB1.
SPECIFIC ACTIVITY
Recognizes human LKB1 at ~51kD. An additional protein was also detected at ~90kD. Species Crossreactivity: mouse and rat
SWISS PROT#
Q15831
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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