Applications:
Suitabe for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000 detected phospho-EGFR (Tyr845) in RIPA lysates from EGF stimulated A431 cells.
Optimal dilution to be determined by researcher.
Positive Control:
EGF stimulated A431 cells
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Applications:
Suitabe for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000 detected phospho-EGFR (Tyr845) in RIPA lysates from EGF stimulated A431 cells.
Optimal dilution to be determined by researcher.
Positive Control:
EGF stimulated A431 cells
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Polyclonal Antibodies
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by immunoaffinity chromatography.
APPLICATION
WB
PHYSICAL FORM
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
SUBCLASS
Receptors, Growth Factor
HOST SPECIES
human
ISOTYPE
IgG
IMMUNOGEN
Synthetic phosphopeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr845). Species Sequence Homology: mouse and rat (100%)
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