Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot Analysis: 0.07-0.4ug/ml of this lot detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 50ng/ml PDGF for 20 minutes.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive...
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot Analysis: 0.07-0.4ug/ml of this lot detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 50ng/ml PDGF for 20 minutes.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Protein Kinases
APPLICATION
WB
CLONE NUMBER
SK703
ACCESSION#
NM_005163
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein A affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG
CROSS REACTIVITY
Bo Ch Hu Mo Rt Xe
IMMUNOGEN
KLH-conjugated, synthetic peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa. The immunizing
sequence is identical in mouse and bovine Akt1. Akt2 and Akt3 share significant homology with the peptide immunogen sequence.
SPECIFIC ACTIVITY
Recognizes human Akt1/PKBa when phosphorylated at Ser473. Crossreactivity to phosphorylated Akt2 and Akt3 likely based on sequence homology. Species Crossreactivity: mouse. Predicted crossreactivity with rat, bovine, chicken, and Xenopus based on immunogen sequence homology.
SWISS PROT#
P31749
COMMODITY CODE
30021010
HOST
rabbit
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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