Cytochrome c is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:2000-1:10,000, do not use dry milk in blocking or dilution buffer in your assay, use BSA (3-5% in blocking and 1-3% in dilution buffers)
Western...
Cytochrome c is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:2000-1:10,000, do not use dry milk in blocking or dilution buffer in your assay, use BSA (3-5% in blocking and 1-3% in dilution buffers)
Western...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Polyclonal Antibodies
SUBCLASS
Enzymes, Cytochrome
APPLICATION
E WB
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified
PHYSICAL FORM
Supplied as a liquid in PBS, 0.02% sodium azide.
GRADE
Purified
ANTIBODY TYPE
Pab
HOST SPECIES
bovine
ISOTYPE
IgY
CROSS REACTIVITY
Bo Hu Mo
IMMUNOGEN
Purified bovine Cytochrome C
SPECIFIC ACTIVITY
Recognizes bovine Cytochrome C. Species Crossreactivity: human and mouse
SWISS PROT#
P62894
COMMODITY CODE
30021010
HOST
chicken
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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