Cytochrome c is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:2000-1:10,000, do not use dry milk in blocking or dilution buffer in your assay, use BSA (3-5% in blocking and 1-3% in dilution buffers)
Western...
Cytochrome c is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:2000-1:10,000, do not use dry milk in blocking or dilution buffer in your assay, use BSA (3-5% in blocking and 1-3% in dilution buffers)
Western...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Polyclonal Antibodies
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified
APPLICATION
E WB
PHYSICAL FORM
Supplied as a liquid in PBS, 0.02% sodium azide.
SUBCLASS
Enzymes, Cytochrome
HOST SPECIES
bovine
ISOTYPE
IgY
IMMUNOGEN
Purified bovine Cytochrome C
ANTIBODY TYPE
Pab
GRADE
Purified
CROSS REACTIVITY
Bo Hu Mo
SPECIFIC ACTIVITY
Recognizes bovine Cytochrome C. Species Crossreactivity: human and mouse
COMMODITY CODE
30021010
HOST
chicken
SWISS PROT#
P62894
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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