ATG5 Antibody: Alkaline Phosphatase

Polyclonal

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Canada

WB, ICC/IF

https://www.ncbi.nlm.nih.gov/gene/9474

NP_001273035.1

Non-hazardous

Non-hazardous

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1 mg/ml

WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Rabbit

Human

Alkaline Phosphatase

Peptide Affinity Purified

ISO 9001:2015

ATG5

Synthetic peptide from the mid-protein of human ATG5

Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.

PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

https://antibodyregistry.org/search.php?q=AB_2704916

Cytoplasm

ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).

1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729. 2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.

Cancer | Apoptosis | Cancer | Autophagy | Cardiovascular System | Heart | Cell Signaling | Metabolism | Metabolism processes

APG5-like Antibody, ASP Antibody, hAPG5 Antibody