Specifications
SHIPPING NOTES
Blue Ice or 4ºC. All orders received in North America by 12:00 PM PST Monday to Thursday will usually be shipped out on the same day. North American customers will typically receive their orders within 1-2 business days. All shipments are F.O.B. from point of shipment, pre-paid and added to the invoice. Freight charges include but not limited to: priority service and packaging materials.Products are shipped with “cold packs” in insulated containers. There is a charge for this packaging and for the shipping costs via FedEx. Please inspect packages immediately upon receipt and inform Labscoop Customer Support of any shortage or damage within 48 hours of receipt. We can only accept returns after we have given prior authorization and issued specific shipping instructions. If a customer wishes to use their own FedEx account number please indicate this on the purchase order or during checkout.
APPLICATION
WB, ICC/IF, FACS, FCM, ELISA, IHC
*USAGE / SAFETY STATEMENT
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
ANTIBODY TYPE
WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); IHC (1:100); optimal dilutions for assays should be determined by the user.
REACTIVITY SPECIES
Species Independent
CONJUGATION/TAG
Unconjugated
PURIFICATION
Protein G Purified
QUALITY CERTIFICATION / COMPLIANCE
ISO 9001:2015
GENE ALIAS
Hexanoyl-Lysine adduct
IMMUNOGEN
Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
SPECIFICITY
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins
STORAGE BUFFER
PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
ANTIBODY ID
https://antibodyregistry.org/search.php?q=AB_2702783
SCIENTIFIC BACKGROUND
Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
REFERENCES
1. Sakai, K et al. (2014) Subcell. Biochem. 77:61-72.
RESEARCH AREA
Cancer | Oxidative Stress | Lipid peroxidation
ALTERNATIVE NAMES
Hexanoyl-Lysine adduct Antibody, HEL (Hexanoyl-Lysine adduct) Antibody, HEL Antibody, HEL Adduct Antibody, Hexanoyl-Lys adduct Antibody, Hexanoyl-Lys Antibody, Hexanoyl-Lysine (HEL) adduct Antibody, Hexanoyl-Lys (HEL) Antibody, Hexanoyl Lysine adduct Antibody, Hexanoyl-Lysine adduct-modified protein Antibody, Hexanoyl-Lysine Adduct (HEL) Antibody