Specifications
SHIPPING NOTES
Blue Ice or 4ºC. All orders received in North America by 12:00 PM PST Monday to Thursday will usually be shipped out on the same day. North American customers will typically receive their orders within 1-2 business days. All shipments are F.O.B. from point of shipment, pre-paid and added to the invoice. Freight charges include but not limited to: priority service and packaging materials.Products are shipped with “cold packs” in insulated containers. There is a charge for this packaging and for the shipping costs via FedEx. Please inspect packages immediately upon receipt and inform Labscoop Customer Support of any shortage or damage within 48 hours of receipt. We can only accept returns after we have given prior authorization and issued specific shipping instructions. If a customer wishes to use their own FedEx account number please indicate this on the purchase order or during checkout.
GENE ID
https://www.ncbi.nlm.nih.gov/gene/2923
*USAGE / SAFETY STATEMENT
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
ANTIBODY TYPE
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
PURIFICATION
Protein G Purified
QUALITY CERTIFICATION / COMPLIANCE
ISO 9001:2015
IMMUNOGEN
Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)
SPECIFICITY
Detects ~57 kDa protein.
STORAGE BUFFER
PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
ANTIBODY ID
https://antibodyregistry.org/search.php?q=AB_2699946
CELLUAR LOCALIZATIONS
Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome
SCIENTIFIC BACKGROUND
ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
REFERENCES
1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.
2. Williams D.B. (2005) J Cell Sci. 119: 615-623
3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889.
4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582.
5. Oliver J.D., et al. (1997) Science 275: 86-88.
6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226.
7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.
8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305.
9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
RESEARCH AREA
Cell Signaling | Protein Trafficking | ER Proteins
ALTERNATIVE NAMES
ERp60 Antibody, ERp61 Antibody, Grp57 Antibody, Grp58 Antibody, P58 Antibody, PDIA3 Antibody, PI PLC Antibody, 58 kDa glucose regulated protein Antibody, 58 kDa glucose-regulated protein Antibody, 58 kDa microsomal protein Antibody, Disulfide isomerase ER 60 Antibody, Disulfide isomerase ER-60 Antibody, Endoplasmic reticulum resident protein 57 Antibody, Endoplasmic reticulum resident protein 60 Antibody, ER p57 Antibody, ER protein 57 Antibody, ER protein 60 Antibody, ERp 57 Antibody, ERp57 Antibody, Glucose Regulated Protein 58 Kd Antibody, GRP 57 Antibody, GRP 58 Antibody, GRP57 Antibody, HsT17083 Antibody, p58 Antibody, PDIA 3 Antibody, PDIA3 Antibody, PDIA3_HUMAN Antibody, Phospholipase C alpha Antibody, PI PLC Antibody, Protein disulfide isomerase A3 Antibody, Protein disulfide isomerase family A member 3 Antibody, Protein disulfide-isomerase A3 Antibody
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