Specifications
SHIPPING NOTES
Blue Ice or 4ºC. All orders received in North America by 12:00 PM PST Monday to Thursday will usually be shipped out on the same day. North American customers will typically receive their orders within 1-2 business days. All shipments are F.O.B. from point of shipment, pre-paid and added to the invoice. Freight charges include but not limited to: priority service and packaging materials.Products are shipped with “cold packs” in insulated containers. There is a charge for this packaging and for the shipping costs via FedEx. Please inspect packages immediately upon receipt and inform Labscoop Customer Support of any shortage or damage within 48 hours of receipt. We can only accept returns after we have given prior authorization and issued specific shipping instructions. If a customer wishes to use their own FedEx account number please indicate this on the purchase order or during checkout.
APPLICATION
WB, ELISA, ICC/IF
GENE ID
https://www.ncbi.nlm.nih.gov/gene/1410
*USAGE / SAFETY STATEMENT
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
ANTIBODY TYPE
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
REACTIVITY SPECIES
Human | Bovine | Pig | Rat
PURIFICATION
Protein G Purified
QUALITY CERTIFICATION / COMPLIANCE
ISO 9001:2015
GENE ALIAS
Alpha B Crystallin
IMMUNOGEN
Native Alpha B Crystallin
SPECIFICITY
Detects ~20kDa (Predicted mol. weight is ~21kDa). Does not cross-react with ?A-crystallin, ?L-crystallin, ?H-crystallin, ?-crystallin, HSP25, HSP27 or HSP47 proteins.
STORAGE BUFFER
PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
ANTIBODY ID
https://antibodyregistry.org/search.php?q=AB_2698566
CELLUAR LOCALIZATIONS
Cytoplasm | Nucleus
SCIENTIFIC BACKGROUND
The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
REFERENCES
1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052.
2. Horwitz J. (1992) Proc Natl Acad Sci USA. 89(21): 10449-10453.
3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490
4. Horwitz J. (2003) Exp Eye Res. 76: 145-153.
5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924.
6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377.
7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215.
8. Yaung J., et al. (2007) Molecular Vision 13: 566-577.
9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.
10. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
RESEARCH AREA
Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Neuroscience
ALTERNATIVE NAMES
AACRYA Antibody, CRYA2 Antibody, CRYAB Antibody, CTPP2 Antibody, HSPB5 Antibody, NY Ren 27 antigen Antibody, alpha crystallin BAntibody