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Histone H3 Antibody (pS10)
SKU / CAT#: V9213-20UG
$21900
usd
$10.95/UG
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V9213-20UG

Histone H3 Antibody (pS10)

NSJ Bioreagents
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SKU / CAT#: V9213-20UG
MPN: V9213-20UG
$21900
usd
$10.95/UG
Available
SHIPS Jul 3rd
Available
SHIPS Jul 3rd
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Description
Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated...
Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Aliquot the phospho-Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This phospho-Histone H3 antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
snilmphospho-histone-h3-antibody-rphh3-6824-v9213
Specifications
CLASS
Primary Antibodies
SUBCLASS
Recombinant Monoclonal
UNIPROT ID
P68431
CLONE NUMBER
rPHH3/6824
*USAGE / SAFETY STATEMENT
This phospho-Histone H3 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human
CLONALITY
Recombinant Mouse Monoclonal
PURIFICATION
Protein A/G affinity
IMMUNOGEN
Amino acids ARK(pS)TGGKAPRKQLc) was used as the immunogen for the phospho-Histone H3 antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
APPLICATION DETAILS
Immunohistochemistry (FFPE): 1-2ug/ml
APPLICATION NOTES
Optimal dilution of the phospho-Histone H3 antibody should be determined by the researcher.
LOCALIZATION
Nucleus, Chromosome
Questions & Answers (0)
Citations (0)
IHC staining of FFPE human bladder tissue with phospho-Histone H3 antibody (clone rPHH3/6824). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder tissue with phospho-Histone H3 antibody (clone rPHH3/6824). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Aliquot the phospho-Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This phospho-Histone H3 antibody is available for research use only.
Regulatory & Compliance
Badge
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