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Biotin / Vitamin B7 / Vitamin H Antibody
SKU / CAT#: V9113-20UG
$21900
usd
$10.95/UG
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  4. Biotin / Vitamin B7 / Vitamin H Antibody
V9113-20UG

Biotin / Vitamin B7 / Vitamin H Antibody

NSJ Bioreagents
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SKU / CAT#: V9113-20UG
MPN: V9113-20UG
$21900
usd
$10.95/UG
Available
SHIPS Jul 7th
Available
SHIPS Jul 7th
SKU
Unit size / Options
Availability
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Description
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the anti-Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This anti-Biotin antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
Mrbysanti-biotin-antibody-spm375-v9113
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
Not Applicable
CLONE NUMBER
SPM375
*USAGE / SAFETY STATEMENT
This anti-Biotin antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Biotin
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the antibody should be determined by the researcher.
LOCALIZATION
target location
Questions & Answers (0)
Citations (0)
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375).
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375).
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the anti-Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This anti-Biotin antibody is available for research use only.
Regulatory & Compliance
Badge
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