Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma...
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the anti-p53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This anti-p53 antibody is available for research use only.
This anti-p53 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2b kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 37-45).
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the antibody should be determined by the researcher.
LOCALIZATION
Predominantly nuclear
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the anti-p53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This anti-p53 antibody is available for research use only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.