This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Safety Statement

This p27Kip1 antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

Uivikp27kip1-antibody-spm348-v9001

Specifications

CLASS

Primary Antibodies

SUBCLASS

Monoclonal

UNIPROT ID

P46527

CLONE NUMBER

SPM348

*USAGE / SAFETY STATEMENT

This p27Kip1 antibody is available for research use only.

FORMAT

Purified

APPLICATION.1

IHC-P

HOST SPECIES

Mouse

ISOTYPE

Mouse IgG1, kappa

REACTIVITY SPECIES

Human

CLONALITY

Monoclonal (mouse origin)

PURIFICATION

Protein G affinity chromatography

IMMUNOGEN

Mouse p27Kip1 protein was used as the immunogen for this p27 antibody.

STORAGE BUFFER

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

APPLICATION DETAILS

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

APPLICATION NOTES

Optimal dilution of the antibody should be determined by the researcher.

LOCALIZATION

Nuclear