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Nucleolin Antibody
SKU / CAT#: V8771-20UG
$21900
usd
$10.95/UG
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V8771-20UG

Nucleolin Antibody

NSJ Bioreagents
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SKU / CAT#: V8771-20UG
MPN: V8771-20UG
$21900
usd
$10.95/UG
Available
SHIPS Jul 1st
Available
SHIPS Jul 1st
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Description
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used...
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Nucleolin antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
gMEENnucleolin-antibody-ncl-7014r-rabbit-monoclonal-v8771
Specifications
CLASS
Primary Antibodies
SUBCLASS
Recombinant Monoclonal
UNIPROT ID
P19338
CLONE NUMBER
NCL/7014R
*USAGE / SAFETY STATEMENT
This Nucleolin antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, WB, IHC-P
HOST SPECIES
Rabbit
ISOTYPE
Rabbit IgG, kappa
REACTIVITY SPECIES
Human. Does not react with Mouse, Rat or Cow. Other species reactivity not known.
CLONALITY
Recombinant Rabbit Monoclonal
PURIFICATION
Protein A affinity chromatography
IMMUNOGEN
Recombinant full length human protein was used as the immunogen for the Nucleolin antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the Nucleolin antibody should be determined by the researcher.
LOCALIZATION
Nucleoli
Questions & Answers (0)
Citations (0)
IHC staining of FFPE human salivary gland with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human salivary gland with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Nucleolin antibody (clone NCL/7014R) as confirmation of integrity and purity.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Nucleolin antibody is available for research use only.
Regulatory & Compliance
Badge
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