PRKCA Antibody / PKC alpha
SKU / CAT#: V8687-20UG
$21900
usd
$10.95/UG
V8687-20UG

PRKCA Antibody / PKC alpha

NSJ Bioreagents
SKU / CAT#: V8687-20UG
MPN: V8687-20UG
$21900
usd
$10.95/UG
Available
SHIPS Jul 3rd
Available
SHIPS Jul 3rd
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Description
Protein Kinase C alpha (PKC) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the isoform of PKC and binds to a sequence at the C terminus of PKC to detect its expression in vitro.
Protein Kinase C alpha (PKC) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the isoform of PKC and binds to a sequence at the C terminus of PKC to detect its expression in vitro.
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
Store the PRKCA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This PRKCA antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
P17252
CLONE NUMBER
133
*USAGE / SAFETY STATEMENT
This PRKCA antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human, Mouse, Rat
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Full-length protein was used as the immunogen for the PRKCA antibody. The epitope has been mapped to amino acids PQFVHPILQSAV at the C terminus.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
APPLICATION DETAILS
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
APPLICATION NOTES
Optimal dilution of the PRKCA antibody should be determined by the researcher.
LOCALIZATION
Cytoplasm, cell membrane, nucleus
Questions & Answers (0)
Citations (0)
IHC staining of FFPE human tonsil with PRKCA antibody (clone 133). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with PRKCA antibody (clone 133). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with PRKCA antibody (clone 133). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the PRKCA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This PRKCA antibody is available for research use only.
Regulatory & Compliance
Badge
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