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EME1 Antibody
SKU / CAT#: V8532-100UG
$49900
usd
$4.99/UG
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V8532-100UG

EME1 Antibody

NSJ Bioreagents
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SKU / CAT#: V8532-100UG
MPN: V8532-100UG
$49900
usd
$4.99/UG
Available
SHIPS Jul 3rd
Available
SHIPS Jul 3rd
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Description
Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3 DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction...
Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3 DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction...
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
Store the EME1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This EME1 antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
NNiPYeme1-antibody-mta31-7h2-1-v8532
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
Q96AY2
CLONE NUMBER
MTA31 7H2/1
*USAGE / SAFETY STATEMENT
This EME1 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
His-tagged recombinant Eme1 of human origin was used as the immunogen for the EME1 antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
APPLICATION DETAILS
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
APPLICATION NOTES
Optimal dilution of the EME1 antibody should be determined by the researcher.
LOCALIZATION
Nucleus
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the EME1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This EME1 antibody is available for research use only.
Regulatory & Compliance
Badge
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