GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit
SKU / CAT#: V8219SAF-100UG
$49900
usd
$4.99/UG
V8219SAF-100UG

GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit

NSJ Bioreagents
SKU / CAT#: V8219SAF-100UG
MPN: V8219SAF-100UG
$49900
usd
$4.99/UG
Available
SHIPS Nov 3rd
Available
SHIPS Nov 3rd
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Description
GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
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Shipped In
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Safety & Storage
Storage Temperature
Store the GCLM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This GCLM antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
P48507
CLONE NUMBER
CPTC-GCLM-1
*USAGE / SAFETY STATEMENT
This GCLM antibody is available for research use only.
FORMAT
Purified (Sodium Azide Free)
APPLICATION.1
WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Recombinant human full-length GCLM protein was used as the immunogen for the GCLM antibody.
STORAGE BUFFER
1 mg/ml in 1X PBS; BSA free, sodium azide free
APPLICATION DETAILS
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
APPLICATION NOTES
Optimal dilution of the GCLM antibody should be determined by the researcher.
LOCALIZATION
Cytoplasm
Questions & Answers (0)
Citations (0)
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
+1
Western blot testing of human 1) A549, 2) A431 and 3) HepG2 cell lysate with GCLM antibody. Expected molecular weight ~31 kDa.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the GCLM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This GCLM antibody is available for research use only.
Regulatory & Compliance
Badge
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