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CD45RA Antibody (Leukocyte marker)
SKU / CAT#: V8043-100UG
$49900
usd
$4.99/UG
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V8043-100UG

CD45RA Antibody (Leukocyte marker)

NSJ Bioreagents
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SKU / CAT#: V8043-100UG
MPN: V8043-100UG
$49900
usd
$4.99/UG
Available
SHIPS Jul 7th
Available
SHIPS Jul 7th
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Description
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA...
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the CD45RA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This CD45RA antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
hsDCjcd45ra-antibody-k4b5-v8043
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
P08575
CLONE NUMBER
K4B5
*USAGE / SAFETY STATEMENT
This CD45RA antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Stimulated human leukocytes were used as the immunogen for the CD45RA antibody.
STORAGE BUFFER
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
APPLICATION NOTES
Optimal dilution of the antibody should be determined by the researcher.
LOCALIZATION
Cell surface, cytoplasmic
Questions & Answers (0)
Citations (0)
IHC staining of FFPE human tonsil with CD45RA antibody (clone K4B5). HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
IHC staining of FFPE human tonsil with CD45RA antibody (clone K4B5). HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
IHC staining of FFPE human spleen with CD45RA antibody (clone K4B5). HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
+1
Flow cytometry testing of human Jurkat cells with CD45RA antibody (clone K4B5); Red=isotype control, Blue= CD45RA antibody.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the CD45RA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This CD45RA antibody is available for research use only.
Regulatory & Compliance
Badge
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