Involucrin Antibody
SKU / CAT#: V7897-100UG
$49900
usd
$4.99/UG
V7897-100UG

Involucrin Antibody

NSJ Bioreagents
SKU / CAT#: V7897-100UG
MPN: V7897-100UG
$49900
usd
$4.99/UG
Available
SHIPS Nov 3rd
Available
SHIPS Nov 3rd
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Description
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and...
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and...
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Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the recombinant Involucrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This recombinant Involucrin antibody is available for research use only.
Regulatory & Compliance
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Specifications
CLASS
Primary Antibodies
SUBCLASS
Recombinant Monoclonal
CLONE NUMBER
rIVRN/827
*USAGE / SAFETY STATEMENT
This recombinant Involucrin antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human
CLONALITY
Recombinant Mouse Monoclonal
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
A recombinant human full-length Involucrin protein was used as the immunogen for this recombinant Involucrin antibody.
STORAGE BUFFER
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
APPLICATION DETAILS
Immunohistochemistry (FFPE): 0.1-0.2ug/ml
APPLICATION NOTES
Optimal dilution of the antibody should be determined by the researcher.
Questions & Answers (0)
Citations (0)
IHC staining of FFPE human skin with recombinant Involucrin antibody (clone rIVRN/827J). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin with recombinant Involucrin antibody (clone rIVRN/827J). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Involucrin antibody (clone rIVRN/827) as confirmation of integrity and purity.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the recombinant Involucrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This recombinant Involucrin antibody is available for research use only.
Regulatory & Compliance
Badge
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