S100B Antibody
SKU / CAT#: V3506-20UG
$21900
usd
$10.95/UG
V3506-20UG

S100B Antibody

NSJ Bioreagents
SKU / CAT#: V3506-20UG
MPN: V3506-20UG
$21900
usd
$10.95/UG
Available
SHIPS Dec 29th
Available
SHIPS Dec 29th
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Description
S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it...
S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This S100B antibody is available for research use only.
Regulatory & Compliance
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Specifications
CLASS
Primary Antibodies
*USAGE / SAFETY STATEMENT
This S100B antibody is available for research use only.
FORMAT
Purified
SUBCLASS
Monoclonal
UNIPROT ID
P04271
CLONE NUMBER
PS1B1-1
APPLICATION.1
IF, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Recombinant full-length human S100B protein was used as the immunogen for the S100B antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Immunofluorescence: 1-2ug/ml,Immunohistology (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
APPLICATION NOTES
Optimal dilution of the S100B antibody should be determined by the researcher.<p>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Cytoplasmic, nuclear
Questions & Answers (0)
Citations (0)
Confocal Immunofluorescent analysis of A2058 cells using Alexa Fluor 488-labeled S100B antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Confocal Immunofluorescent analysis of A2058 cells using Alexa Fluor 488-labeled S100B antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).
IHC testing of FFPE human melanoma with S100B antibody (clone PS1B1-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
+1
IHC testing of FFPE human schwanoma with S100B antibody (clone PS1B1-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This S100B antibody is available for research use only.
Regulatory & Compliance
Badge
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