ZP2 Antibody
SKU / CAT#: R32192
$41900
usd
$4.19/UG
R32192

ZP2 Antibody

NSJ Bioreagents
$41900
usd
$4.19/UG
Available
SHIPS Jul 7th
Available
SHIPS Jul 7th
SKU
Unit size / Options
Availability
Price
No products found
Description
Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing...
Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
After reconstitution, the ZP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Safety Statement
This ZP2 antibody is available for research use only.
Regulatory & Compliance
Badge
Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Polyclonal
UNIPROT ID
Q05996
*USAGE / SAFETY STATEMENT
This ZP2 antibody is available for research use only.
FORMAT
Antigen affinity purified
APPLICATION.1
WB, IHC-P, IHC-F
HOST SPECIES
Rabbit
ISOTYPE
Rabbit IgG
REACTIVITY SPECIES
Human
CLONALITY
Polyclonal (rabbit origin)
PURIFICATION
Antigen affinity
IMMUNOGEN
Amino acids ENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDD of human ZP2 were used as the immunogen for the ZP2 antibody.
STORAGE BUFFER
0.5mg/ml if reconstituted with 0.2ml sterile DI water
APPLICATION DETAILS
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
APPLICATION NOTES
Optimal dilution of the ZP2 antibody should be determined by the researcher.
LOCALIZATION
Cytoplasmic
Questions & Answers (0)
Citations (0)
Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with ZP2 antibody. Expected/observed molecular weight ~82 kDa.
Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with ZP2 antibody. Expected/observed molecular weight ~82 kDa.
IHC testing of FFPE human intestine cancer tissue with ZP2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placental tissue with ZP2 antibody.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
After reconstitution, the ZP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Safety Statement
This ZP2 antibody is available for research use only.
Regulatory & Compliance
Badge
Science SamplesWe’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.

Join Our List

mail
Subscribe to the Scoop blog and get the latest updates on life in the lab.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Related Blog Articles
Related Blog Articles