Specifications
SHIPPING NOTES
Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).
APPLICATION
WB, ICC/IF, IHC
*USAGE / SAFETY STATEMENT
This product is for research use only. It is not intended for diagnostic or therapeutic use.
CONJUGATION/TAG
Unconjugated
PURIFICATION
Immunogen affinity purification.
DILUTION
WB: 1:1,000-1:5,000, ICC/IF: 1:1,000-1:5,000, IHC: 1:1,000-1:5,000
IMMUNOGEN
Recombinant Aequoria coerulescens GFP protein, expressed in and purified from E. coli.
STORAGE BUFFER
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
SEQUENCE
MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFGFVTAAAITHGMDELYK
GENERAL NOTES
This antibody can be used to verify the expression, size, and stability of both AcGFP and eGFP fusion proteins in western blotting experiments, and to amplify GFP signals in tissues of transgenic animals.